The Evaluation of Leukocyte-Depleted Pool Platelet Concentrates (LDPPC) Quality: Prepared from 3 Interim Platelet Units

Abstract

Background and Objective: The leukocyte-depleted pool platelet concentrates (LDPPC) were prepared by combining four units in the same ABO blood group of interim platelet unit (IPU) and adding the platelet additive solution (PAS) 280 ml, then filtered out of white blood cells by a pooling set of Reveos^® System. To obtain the platelet yields (PY) more than 240 x10⁹ cells per unit, the total platelet yields index (PYI) of 4 IPUs were counted to get 270 x10⁹ cells or more by using the loss of platelets value during the filtering process (approximate to 30 x10⁹ cells), plus the standard value (240 x10⁹ cells) to create the LDPPC that reached the international standards. Nowadays, the LDPPC that produced by this technique passed the benchmark 100%. From daily work, it was found that sometimes just 3 IPUs can give a PYI value ≥ 270 x 10⁹ cells. The research team intended to study whether combining just 3 IPUs according to the study criteria would be able to produce LDPPC that reached the international standards or not.

Methods: To produce the LDPPC (N= 30), the collected blood bags were centrifuged by the automatic machine. The bag number, volume, and PYI value of the IPU estimated by the automatic machine were recorded in the recording form. The 3 IPUs with the same ABO blood group and combined PYI value of ≥ 270 x 10⁹ cells were pooled and added 280 ml of PAS, then filtered to remove white blood cells to obtain LDPPC. The LDPPC was gently shaken with a shaker for 15 minutes, then it was sampling 0.5 ml for testing the complete blood count (CBC) by counting with the Sysmex Hematology Analyzer model XN-550 and the contaminated white blood cells were counted by the ADAM-rWBC machine. The results were analyzed and compared them with the international standards.

Results: The results showed that the average volume of LDPPC was 344.20 ml, white blood cell contamination was 0.19 X 10⁶ cells per unit and platelet yields were 235.76 X 10⁹ cells per unit.

Conclusion: The LDPPC productions by new method reached the recommended quality of Council of Europe (EU) 96.67% (29/30) and reached the American Association of Blood Banks (AABB) 43.33% (13/30).