Production of Purified Chick Embrio Cell Rabies Vaccine

Abstract

The production of PCEC rabies vaccine had been performed by the following method. The primary chick embryo cell was cultured from the 7-day old chick embryonated eggs and rabies virus, Hep-flurry strain was inoculated into this cell culture. On the6^th, 11 ^th and 16 ^th day after inoculation, the culture fluid was harvested. The crude viral suspension must have HA titer not less than 1:32 Then viral suspension fluid wasinactivated by adding 1:4000 B-propiolactone. The inactivated virus suspension was concentrated and purified by ultrafiltration and ultracen-trifugation, respectively. The purified virus suspension was then diluted to have final HA titer at least 1:256. Finally, The vaccine was Iyophilized and the quality control tests of the lyophilized vaccine were performed according to WHO requirements for Rabies Vaccine for Human Use. Fourty-four hundred doses (4,400 doses) of the vaccine produced passed all quality control tests.