The Antibiofilm activity, Cytotoxicity and Synergy of polymyxin B mixed with Sterculia foetida L
Abstract
This research was aimed to study antibiofilm activity, cytotoxicity and synergistic effect of ethanol extract from stem bark of Sterculia foetida L. in combination with polymyxin B against some drug resistant bacteria. As a result of screening antibiofilm activity, stem bark of Sterculia foetida were be able to inhibit biofilm formation of Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 2592, and Pseudomonas aeruginosa ATCC 27853 at the times of 4-24 hours after inoculum. Also, Sterculia foetida showed the best anti-biofilm activity against Staphylococcus aureus ATCC 25923 at 24 hours after inoculum and showed % inhibition as 73.0 1±1 6.68 %. Additionally, synergistic effect of Sterculia foetida in combination with polymyxin B was reported that using 1/2 MIC of Sterculia foetida mixed with 1/2 MIC of polymyxin B enhanced antibacterial activity against drug resistant Acinetobacter baumannii and Pseudomonas aeruginosa by time kill assay. Finally, our discovery was indicated that stem bark of Sterculia foetida showed no toxicity against macrophage (RAW 264.7). However, Sterculia foetida significantly increased cell viability of macrophage when treated the macrophage with 31.25 - 1,000 µg/ml of Sterculia foetida extract (p<0.01).
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