The Effect of Moringa oleifera Lam. Leaves Extract on IL-6 in Inflammatory Human Macrophages Induced by Lipopolysaccharide
Keywords:
cytokine, Moringa oleifera, macrophage, lipopolysaccharide, anti-inflammationAbstract
Inflammation is a result of immune mechanism response to foreign organism and infection. It causes by pro-inflammatory cytokines released from the immune system such as tumor necrotic factor-α (TNF-α), interleukin (IL)-1β and IL-6. Non-steroidal anti-inflammatory drugs (NSAIDS) and steroid are the most common drugs for inflammation treatment. However, using of this medication can cause side effects. The objective of this study was to investigate the anti-inflammatory property of Moringa oleifera leaves extract on human monocyte-derived macrophages induced by lipopolysaccharide of Gram-negative bacterial cell wall. Gene expression of IL-6 was evaluated by quantitative reverse transcription polymerase chain reaction. IL-6 was measured by enzyme-linked immunosorbent assay. The results indicated that post-treatment of inflamed cell with Moringa leaves extract significantly reduced both IL-6 gene expression and IL-6 cytokine level (p<0.05) compared to the control groups. This study demonstrated that Moringa oleifera leaves extract reduced IL-6 in macrophages induced by lipopolysaccharide. This plant should be further investigated and the knowledge could be applied for the development of anti-inflammation drugs.
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