DEVELOPMENT OF EYE IRRITATION TEST BY USING THE ISOLATED CHICKEN EYE TEST, LAC-THAI NIH
Keywords:
eye irritation test, solated Chicken Eye tes, ocular irritation index, histopathological eye effectAbstract
Laboratory Animal Center Thai NIH has developed the Isolated Chicken Eye (ICE) eye irritation test method to meet the criteria of OECD Test Guideline No.438, which is the test for identifying safety and classify irritants. This assessment is evaluated the ICE test with ocular irritation index and histopathological eye effect in corneal chicken. Results from a total of 26 chemicals categorized by the United Nations Globally Harmonized System of Classification and Labelling of Chemicals conducted with ICE test showed that the ocular irritation index and corneal opacity value obtained from Category 2A (irritant) and 2B (mild irritant) substances had statistically significant when compared to Category 1 (severe irritation) substances. While the score of corneal epithelial necrosis obtained from Category 2A and 2B substances were significant finding when compared to Category 1 substances. Therefore, the evaluation of the irritant index in combination with the histopathology for ICE test will increase the test efficiency.
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