Detection of Sodium Hydrosulfite and Escherichia Coli Contaminated in Bean Sprout from the Market in Bang Phli District, Samut Prakan Province

Abstract

Bean sprouts are the sprouts of mung bean seeds. It is a nutritious vegetable, containing protein, B vitamins, vitamin C, dietary fiber, mineral salt and low calories. Most people like to eat fresh or as a side dish to eat with food. There may be contaminated with bleach because manufacturers want the sprouts to be white and always fresh. Furthermore, it has been reported that it can also be contaminate with pathogenic microorganisms Escherichia coli (E. coli), a Gram-negative bacterium, have a short rod shaped and non-spore. E. coli live in intestine of homoeothermic animal and humans. E. coli has been regarded as a biological indicator of fecal contamination. Therefore, it was used as an index of fecal contamination from the announcement of the Ministry of Public Health in ๒๐๑๘ on food additives prescribed to use sulfite bleach is required in certain foods in limited quantities, while sodium hydrosulfite is prohibited in foods. E. coli contaminated in ready-to-eat fresh vegetables must less than ๑๐๐ MPN/g which informed to notification of announcement of the Department of medical sciences on the microbiological quality criteria of food and food contact containers, No. ๓, in ๒๐๑๗. The objective of this study was to detect sodium hydrosulfite and detect E. coli contaminants in bean sprouts from the Market in Bang Phli District, Samut Prakan Province. Thirty samples were tested for bleach using a sodium hydrosulfite test kit and detect coliform bacteria and E. coli by standard methods of Bacteriological Analytical Manual (BAM) ๒๐๒๐ using the method for determining MPN/g, ๓ tubes system. The results of the experiment revealed that no sodium hydrosulfite contamination was found in all the bean sprouts of the sample. The range of E. coli contaminants is between less than <๓.๐ ถึง ๒๓ MPN/g which is within the specified standard of E. coli less than ๑๐๐ MPN/g. Therefore, the bean sprouts in Bang Phli District markets can be safe for consumption.