Hemorrhoid Remedy from Cissus Quadrangularis Linn - ยารักษาโรคริดสีดวงทวารจากสมุนไพร เพชรสังฆาต

Abstract

                The stem of Cissus quadrangularis L.  has been used as traditional medicine for hemorrhoid.  Currently, in drug formulation, it is prepared by pulverizing dried stems resulting in dubious quality of the products and contaminations.  Herbal medicine quality is influenced by several factors, such as when and where the raw materials were collected and the shelf-life of herbal medicine.  Furthermore, the microbial contamination and the quantities of calcium oxalate in the raw materials of C. quadrangularis are also causes of concern to patients.  In this study, the extract formulation of C. quadrangularis remedy was developed.  Crude extract of the stem of C. quadrangularis was prepared by extraction with 95% ethanol.  The hexane extract and the ethanol extract were obtained from subsequent liquid/liquid extraction of the crude extract.  The anti-inflammatory activities of these extracts were investigated employing the ethylphenyl propiolate (EPP)-induced ear edema in mouse model at various concentrations of 0.1, 1 and 5% in EPP solutions and using indomethacin as positive control.  The hexane extract (1% concentration) elicited the significant (p<0.05) inhibitory effect on the ear edema formation at 30, 60 and 120 minuits after hexane extract-EPP-treatment.  The crude extract (5% concentration) also significantly inhibited ear edema over the period 30 minuits, while the ethanol extract did not produce the inhibition.  The potent hexane extract was further isolated and a purified compound was obtained.  The structure elucidation of this compound led to lupeol.  The hemorrhoid remedy formulation was developed by using 1% hexane extract in fatty base.  The stability of the remedy was evaluated after being stored at 8-15oc for 3 months.

Key words:  Cissus quadrangularis L., hemorrhoid remedy, anti-inflammatory activity, lupeol