Development and Validate Method for Zoonoses Test Kits

Abstract

Since zoonoses or vector-borne diseases cause public health problems as emerging-reemerging diseases. Therefore, we developed and evaluated multidot-IFA for detecting antibody against brucellosis/ melioidosis/leptospirosis/scrub typhus/murine typhus and three test kits of multiplex real-time PCR for detecting brucellosis/leptospirosis/melioidosis/tularemia, brucellosis/leptospirosis/Q fever/scrub typhus, or bartonelloses/murine typhus/rickettsioses/scrub typhus within one reaction. By evaluation, we found the stability of multidot-IFA slide could be sustained at -20°C for at least 12 months with the sensitivity was ranging from 86-100%. Whereas multiplex real-time PCR could detect pathogenic bacteria without cross reaction at Ct cut-off 33±1 which limit of detection was varied from 0.01 pg to 1,000 pf. Herein, we concluded that multidot-IFA and multiplex real-time PCR were highly sensitive and specific for the diagnosis of multiple bacterial infection within one reaction. Thus these test kits could be less time con-suming and cost effective for the surveillance and control of emerging zoonoses.