Proficiency Testing of Dengue IgM and IgG Detection in Serum by Immunochromatography Rapid Test

Abstract

The Regional Medical Sciences Center 1 Chiang Mai has established the proficiency testing (PT) program of Dengue antibody IgM and IgG detection in serum by immunochromatography rapid test during 2013-2016. There were 31 medical laboratories in Northern part participated and returned test results. Sample preparation in this program was pooled sera and tested for homogeneity and stability throughout the specified period that participants enrolled used as PT samples. Evaluation of laboratories was conducted by using target value derived from confirmation antibody Dengue IgM and IgG results by antibody capture ELISA and rapid test. The results of participantng labiratories were compared with target value in terms of accuracy and precision. The criteria of this program was set, laboratories which have the percentage of concordance 90.0-100.0 % were considered excellent, 80.0-89.9% were good, 70.0- 79.9% were satisfactory and less than 70% were unsatisfactory. The results of laboratory evaluation had shown the percentage of the average accuracy of dengue IgM and IgG detection as excellent 51.6%, good 41.9% and satisfactory 6.5%. Whereas, the average precision of dengue IgM and IgG detection was at the excellent level 83.9%, good 9.7% and satisfactory 6.4%. These indicated most laboratories were at excellent level. In conclusion, proficiency testing program plays an important role in the development of laboratory capability to be effective in accordance with the quality management system.